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We would like to show you a description here but the site won't allow us.The in vitro coevolution method involves design of a hypothetical pathway for the target function followed by stepwise directed evolution of the corresponding protein along the pathway. As a test case, this strategy was used to engineer variants of human estrogen receptor α ligand-binding domain (hERαLBD) with novel corticosterone activity.In examining these questions, we begin by considering the continuing relevance of one of the earliest analyses of protein evolution, performed >40 years ago by the great chemist Linus Pauling and his colleague Emile Zuckerkandl ().Working at the time when it was first becoming feasible to obtain amino acid sequences, Pauling and Zuckerkandl assembled the sequences of hemoglobin and myoglobin ...…

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